ISSN (Online) : 2277-4572
Author(s): Naga Sirisha M and Shanta Kumari A
A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous estimation of cinnarizine (CIN) and domperidone (DOM) in tablet dosage form. The method was carried out on a C-18 (250mm × 4.6mm i.d, 5µm) column with a mobile phase consisting of methanol and acetonitrile in the ratio (70:30 v/v) and flow rate of 1.0 mL min-1 . The detection was carried out at 270 nm. The retention time for CIN and DOM were found to be 3.389 and 4.793 min respectively. The CIN and DOM followed linearity in the concentration range of 40-160 μg mL-1 and 45- 105 μg mL-1 with r2 = 0.999. The amount of both drugs estimated by the proposed method was found to be in good agreement with label claim. The developed method was validated for precision, accuracy, sensitivity, robustness and ruggedness. The developed method can be used for routine analysis of titled drugs in combination in the tablet formulations.
