ISSN (Online) : 2277-4572
Author(s): Deshpande Padmanabh B., Butle Santosh R.
A new simple, accurate, precise and selective stability- indicating high performance thin layer chromatographic (HPTLC) method has been developed and validated for simultaneous estimation of Atazanavir and Ritonavir in combined tablet dosage form. The mobile phase selected was Toluene: Ethyl acetate: Methanol (6: 4.5: 0.6, v/v/v) with UV detection at 240 nm. The retention factor for Atazanavir and Ritonavir were found to be 0.25 ± 0.004 and 0.41 ± 0.004. The method was validated with respect to linearity, accuracy, precision and robustness. The drugs were subjected to stress condition of hydrolysis (acid, base), oxidation, photolysis and thermal degradation. Results found to be linear in the concentration range of 1000-8000 ng band-1 for Atazanavir and 500-4000 ng band-1 for Ritonavir respectively. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. The % assay (Mean ± S.D.) was found to be 100.40 ± 0.964 for Ritonavir and 99.59 ± 1.103 for Atazanavir.
