SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF METFORMIN HCL AND FENOFIBRATE IN THEIR SYNTHETIC MIXTURE

Abstract

Author(s): Bharat G. Chaudhari*

Two simple spectrophotometric methods have been developed for simultaneous estimation of Metformin HCl and Fenofibrate from their synthetic mixture. Method-I involved simultaneous equation method and Method-II is the Q-absorbance method. For simultaneous equation method, the absorbances of the standard solutions were taken at two wavelengths 237 nm (λ-max of Metformin HCl) and 288 nm (λ-max of Fenofibrate). For Q-absorbance method, the absorbances of the standard solutions were taken at two wavelengths 237 nm (λ-max of Metformin HCl) and 249 nm (Isoabsorptive point), in methanol. Linearity range was found to be 3-20 μg/ml for Metformin HCl and Fenofibrate in both methods based on the ratio of the two drugs in combined dosage form. The accuracy and precision of the methods were determined and validated statistically. Both methods showed good reproducibility and recovery with RSD less than 2. Proposed methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of Metformin HCl and Fenofibrate in pharmaceutical dosage form.