MUTATION ANALYSIS OF K RAS GENE IN COLORECTAL CANCER IN IRAQI PATIENTS

Abstract

Author(s): Haidar J. Muhammed *, Ali H. Al – Saadi, Saad Ali

The high frequency of Kras mutations and the observation that they mostly appear during early stages of tumor progression provide strong argument supporting a causative role of Kras in human tumorigenesis. A fragment of Kras gene with molecular size 108bps, amplified by conventional PCR was detected in extracted of blood genomic DNA from (29) patients with colorectal cancer (CRC) collected from teaching hospitals of some Iraqi governorates. The patients average aged was 46.86 years, male to female ratio was 65.5% to 34.5% respectively. The sample of the patients were compared with 4 healthy as a control which were processed for detection of mutations and confirmed by PCR-Sequences. The sequences analysis of the variant regions for the amplification of the defect gene were 20.68% (6 out of 29) of the patients as compared with the Sequences of Gene Bank.