Author(s): J. Vijayakumari and T. Leon Stephan Raj*
The present study was carried out to characterize the bioactive constituents present in aerial parts of acetone and chloroform extract of Dicranopteris linearis (Burm.f.) Underw. using UV-VIS, FTIR and HPLC. UV, FTIR and HPLC spectroscopy is an established time-saving method to characterize and identify functional groups. There phytoconstituents were analyzed for their phytochemical properties, High Performance Liquid Chromatography (HPLC) assay, Fourier Transform Infra-Red (FTIR). The UV-VIS profile showed different peaks ranging from 330-800 nm with different absorption respectively. FTIR analysis and UV-VIS analysis showed the presence of flavonoids and a phenolic compound. The FTIR spectra had amply evidenced the occurrence of OH group together with the Terpenoids and Phenol. The FT-IR spectrum showed the presence of an amine (N-H), alkyl (C-H), nitrile (C≡N), halo formyl (C=O), alkenyl (C=C), hydroxyl (O-H), haloalkane (C-F), nitrates and carbonate compounds. Totally six phyto constituents were separated and identified in HPLC analysis. The results confirm the fact that this plant posse’s important bioactive constituent, so further scientific investigation is needed to isolate the pure compounds from the plant.